Learning Objectives:

1. To know how to determine the concentration of DNA, given the absorbance at 260 nm. (not in the text, but see my lecture notes)

2. To know how gel electrophoresis can be used to resolve DNA molecules of different sizes, and how restriction enzymes can be used to make fragments of specific sizes; to know how to do a Southern blot, and how PCR can be used to amplify a specific fragment. (pages 199-207).

3. To understand the different methods for sequencing DNA, and the advantages and roughly the speed and practicality of each.

Last modified on: 1 February, 2000 by Dave Ussery